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In many situations, baseline separation on the peaks can be realized only with gradient elution and low column loadings. As a result, two downsides to elution manner chromatography, Primarily within the preparative scale, are operational complexity, resulting from gradient solvent pumping, and lower throughput, resulting from small column loadings.
Allows simultaneous and ongoing operation of up to 3 chromatography separations. These might be Element of a batch and/or multi-column approachEnables better productiveness than regular chromatography, minimized buffer and resin volumes and decreased resin chargesAn ineffective HPLC column can influence your Assessment, resulting in inaccurate resu
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